Seth Green

as The Nerd / Batman / Robin / Aquaman / The Penguin / Martian Manhunter / Toyman / Scarecrow / Gorilla Grodd / Little Cheese / Swamp Thing / Abin Sur / Ganthet / Green Arrow / Hawkman / Jimmy Olsen / Various (voice)

Breckin Meyer

as Superman / Mirror Master (voice)

Nathan Fillion

as Green Lantern / Mr. Freeze (voice)

Alex Borstein

as Wonder Woman / Giganta / Woman (voice)

Matthew Senreich

as The Flash / Chillblaine / Brainiac / Wildcat (voice)

Alfred Molina

as Lex Luthor / Firestorm / Mr. Banjo (voice)

Paul Reubens

as The Riddler (voice)

Neil Patrick Harris

as Two-Face / Black Manta (voice)