This volume�comprehensively covers�new technologies�and methodologies that have appeared for the study of mouse development. �� This volume is Part B�of an update�of volume 225, Guide to Techniques in Mouse Development, edited by P. M. Wassarman and M. L. DePamphilis and published in 1993. � Comprehensively covers�new techniques for the cryopreservation of gametes and embryos, production of transgenic and null (knockout) animals (use of ES cells), generation of conditional/inducible mutant animals, use of gene-trap