Methods in Plant Biochemistry, Volume 10: Molecular Biology covers techniques for working with plant genes, specific techniques for modifying the genetic contents of plant cells, and applications of molecular biology techniques to understanding how plants work. The book discusses RNA extraction and fractionation; in vitro translation of plant messenger RNA; and cDNA cloning and screening. The text also describes nucleic acid blotting and hybridization; applications of protein blotting in plant biochemistry and molecular biology; and the applications of polymerase chain reaction. Non-radioactive in situ RNA hybridization using digoxigenin and an application for co-localization studies with radioisotopes are also considered. The book further tackles the immunolocalization of antigens in plants with light and transmission electron microscopy; protoplast fusion; and the import of in-vitro synthesized proteins into intact chloroplasts and isolated thylakoids from higher plants. The text also looks into seed development and the molecular and genetic analysis of tomato fruit development and ripening. Molecular biologists, geneticists, and people involved in plant genetic manipulation, horticulture, and agriculture will find the book invaluable.
